Additional file 4 of Establishment of a transdermal infection model with Leishmania amazonensis

Additional file 4: Figure S3. Configuration of 1.5-mm depth is not optimal for the transdermal model of infection. Female BALB/c mice aged 6-8 weeks were infected with 2x106 Leishmania amazonensis promastigotes in the stationary phase, either by transdermal route (TD) with different microneedle depth (1.5mm and 1.0mm). Mice lesion thickness and macroscopic aspect were assessed over 49 days, after which the animals were euthanized for Limited dilution assay. (A) Lesion thickness in millimeters per days of infection; (B) Representative ear lesion photographs; (C) Quantification of parasite load per tissue gram of the ear; (D) and draining lymph node. The data (means ± standard deviations; n = 5) are representative of two independent experiments producing the same result profile.