Additional file 5 of Establishment of a transdermal infection model with Leishmania amazonensis

Additional file 5: Figure S4. Configuration of 1.5-mm depth caused tissue destruction. Female BALB/c mice aged 6-8 weeks were infected with 2x106 Leishmania amazonensis promastigotes in the stationary phase, either by transdermal route (TD) with different microneedle depth (1.5mm and 1.0mm). Weekly photographs of mice lesion macroscopic aspect was assessed over 49 days. The data (means ± standard deviations; n = 5) are representative of two independent experiments producing the same result profile.