lncRNAs Transcriptomics Elucidates the Potential Mechanism of Naoshuantong Capsule in Alleviating Synaptic Dysfunction in a murine model of Cerebral Ischemia/Reperfusion Injury

Mice were randomly allocated to three study groups: (1) sham group, (2) model group, and (3) model + naoshuantong group (NST group, 0.468 g/kg). On day 0, all groups except the sham group were subjected to middle cerebral artery occlusion (MCAO). At 2 hours after reperfusion following MCAO, the NST group of mice was orally administered naoshuantong capsule (0.468 g/kg, via gastric gavage, prepared in 0.5% sodium carboxymethyl cellulose [Na-CMC]), while receiving an equal volume of saline via intraperitoneal injection. Total RNA was extractedfrom cerebral cortices of mice in sham, model, and NST groups(n = 6/group) usingTRIzol reagent (Invitrogen). Transcriptome profiling employed the Agilent Mouse Competing Endogenous RNA Microarray 2019 (4*180K, Design ID: 086242) across all 18 samples.