Additional file 3 of Spatiotemporal transcriptomic mapping reveals region-specific glial activation and astrocyte shifts in epileptogenesis beyond the hippocampus

Supplementary Material 3: Supplementary Fig. 3. Sub-clustering of the cortex. A Four panels of UMAP representation of cortical cluster spots colored according to subcluster, condition (treated or control), time points or sample of origin (from top to bottom and from left to right). The mean silhouette score is indicated in the top of each plot. B Spatial plots of sub-clusters on tissue slices of samples at the D5 time point (treated samples on top, control samples on bottom). C and D Example of cortical layers 6a and 5 marker (Mef2c and Rprm) expression levels after log2 transformation. Violin plots per cluster (top), spatial plots on the tissue slice of the control samples at the D5 time point (bottom).