Additional file 1 of OGT-mediated O-GlcNAcylation of MAGI1 exacerbates high glucose-triggered inflammation and dedifferentiation of vascular smooth muscle cells by activating the PI3K/AKT pathway

Supplementary Material 1. Supplementary Figure 1: The effect of site-directed mutation on O-GlcNac level and cell function. (A) O-GlcNac protein was detected in VSMCs treated with HG, HG+MAGI1-WT, HG+MAGI1-S991A, HG+MAGI1-S1362A, and HG+MAGI1-DM. (B-D) VSMCs treated with HG, HG+MAGI1-WT, HG+MAGI1-S991A, HG+MAGI1-S1362A, and HG+MAGI1-DM were checked for cell viability (B), migration (C), and the protein levels of PI3K, p-PI3K, AKT, and p-AKT (D). *P < 0.05, **P < 0.01, ***P < 0.001.