Additional file 2 of O-GlcNAcylation expression predicts a favorable prognosis and mitigates malignant phenotypes via MYCN suppression in neuroblastoma

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Authors: Neng‐Yu Lin, Hsiu-Hao Chang, Chia-Yeh Hsieh, Hsiu-Ling Chang, Wan-Ling Ho, Yen-Lin Liu, Pei‐Yi Wu, Chi‐Tai Yeh, Min‐Chuan Huang, Wen-Ming Hsu
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Abstract

Supplementary Material 2. Supplementary Fig. 2: Quantitative analysis of immunohistochemical staining in Th-MYCN transgenic mice tumors.Quantification of (A) O-GlcNAc, (B) MYCN, and (C) GAP43 expression levels in tumor tissues from control and Thiamet G-treated Th-MYCN mice (corresponding to the representative images in Fig. 5E). Data are presented as the mean percentage of positive cells (or H-score) ± SD (n = 6 per group). Thiamet G treatment significantly increased O-GlcNAc and GAP43 levels while reducing MYCN expression compared to the control group. **p < 0.01 (Student’s t-test).

Fields of Study & Subjects

Neuroblastoma Research and Treatments Glycosylation and Glycoproteins Research Glioma Diagnosis and Treatment

Publication details

Journal / source
Figshare
Publication date
1 January 2026
Language
EN