Additional file 5 of Paclitaxel induces trained immunity via the GPR183–STING axis to enhance host defense against MRSA infection

Additional file 5. Macrophages derived from PTX-trained mice exhibit enhanced functional state after the secondary stimulus. (A) Experimental scheme. (B) The expression of MHC class II on macrophages at 7 days post-PTX exposure. (C, D) NO and TNF-α production was detected in the supernatants following LPS stimulation. (E, F) Phagocytosis and killing capacity were measured by counting CFU. Data are presented as mean ± SEM (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001.