Additional file 3 of Experimental insights in taxon-specific functional responses to droughts in glacier-fed stream biofilms

Supplementary Material 3: Figure S1. Quality and characteristics summary of bacterial MAGs recovered from a glacier-fed stream biofilm growth experiment under repetitive droughts. Figure S2. Eukaryotic MAGs recovered from a glacier-fed stream biofilm growth experiment under repetitive droughts. Figure S3. Microbial composition of whole glacier-fed stream biofilm metagenomes. Figure S4. Successional patterns in glacier-fed stream biofilm microbiomes under repetitive droughts. Figure S5. Taxonomy and successional patterns of DNA and RNA viral MAGs in glacier-fed stream biofilm microbiomes under repetitive droughts. Figure S6. Drought-induced changes in MAG activity (metatranscriptome) of glacier-fed stream biofilms. Figure S7. Drought-induced changes in activity (whole metatranscriptome) in glacier-fed stream biofilms. Figure S8. Drought-induced changes in glacier-fed stream biofilm MAGs’ activity. Figure S9. Principal component analysis (PCA) plot of variance-stabilizing transformed counts, showing clustering of replicate samples. Figure S10. Differential abundance analyses showing the taxon-scaled differentially expressed genes between pre- and post-drought samples. Figure S11. Drought-induced relative changes in MAGs’ activity. Figure S12. Number of drought-induced DEGs per KEGG metabolic category for each drought period. Figure S13. Drought-induced relative changes in biofilm functionality (KEGG subcategories). Figure S14. Drought-induced relative changes in biofilm functionality (KEGG pathways). Figure S15. Gene orthologs associated with carbon fixation via the Calvin cycle in phototrophic MAGs. Figure S16. Gene orthologs associated with photosynthesis in phototrophic MAGs. Figure S17. Gene orthologs associated with photosynthetic antenna proteins in phototrophic MAGs.