Additional file 4 of Nexilin promotes calcium-dependent endo-lysosomal fission required for retrograde transport

Supplementary Material 4: Figure S4. Calcium accumulation in LE/Lys is rescued by reintroduction of nexilin. A U2OS cells treated with control siRNAs, siRNAs against nexilin, or siRNAs against nexilin and then transfected with siRNA-resistant BFP-nexilin, were transfected with mCherry-Rab7b and incubated with Fluo-4 AM for 30 minutes before live cell imaging. Scale bar: 10 µm. B Intensity map showing Fluo-4 AM intensity for the cells shown in A). Scale bar: 10 µm. C Average mean intensity of Fluo-4 AM fluorescence in LE/Lys over the cytosol. Data represents mean ± s.d. from three independent experiments (n = 60 cells in total per condition). One-way ANOVA followed by a Tukey’s post hoc test was applied for statistical analysis. ns non-significant, ** p < 0.01