Additional file 2 of Toxoplasma gondii drives myeloid immune cell recruitment to amyloid plaques in Alzheimer’s model mice

Supplementary Material 2: Sup. Fig 1: Amyloid and IBA1+ cells in the subiculum T. gondii-infected 5xFAD animals. Three-month-old 5xFAD mice were injected i.p. with PBS or type II T. gondii, and amyloid and IBA1+ cells were analyzed at 6 wpi. A) Representative images show amyloid (Amylo-Glo) signal in the subiculum of the hippocampus of PBS or T. gondii-injected mice. B) Average percent of amyloid area per FOV in each mouse. C) Average volume of amyloid plaque cores per FOV. D) Average numbers of amyloid plaques per FOV. E-F) Average percent of CD68 signal per FOV (E) and (F) CD68+amyloid+ signal per FOV in the. G-I) Average IBA1 (G), CD68 (H), and IBA1+CD68+ signal per FOV. J) IBA1 volume within a 15 µm radius of plaque cores was normalized to total plaque core volume per FOV in each animal. K-L) CD68 colocalization with amyloid (K) and in IBA1+ cells (L) per FOV in each animal. For B-L, n= 6-7 mice per group. Student’s t test, * p