Additional file 2 of NSUN2 restrains gastric cancer cell apoptosis and ferroptosis by promoting the m5C modification of EPYC

Supplementary Material 2: Supplementary Fig. 2. Effects of sh-EPYC and sh-NSUN2/oe-EPYC on lipid ROS and ACSL4 level. (A) C11-BODIPY probe and (B) WB were used to measure lipid ROS and ACSL4 levels in AGS cells transfected with sh-NC/sh-EPYC (n = 3). Lipid ROS and ACSL4 levels in AGS cells transfected with sh-NC/sh-NSUN2/oe-EPYC were determined by (C) C11-BODIPY probe and (D) WB (n = 3). A-D, Student’s t-test. All experiments were performed in triplicate.