Additional file 3 of NSUN2 restrains gastric cancer cell apoptosis and ferroptosis by promoting the m5C modification of EPYC

Supplementary Material 3: Supplementary Fig. 3. MeRIP results and the effects of oe-NSUN2/sh-EPYC on GC progression. (A-B) MeRIP assay was used to analyze sh-NSUN2-WT/MUT and sh-NSUN2+oe-EPYC-WT/MUT on the m5C level of EPYC (n = 3). (C) Colony formation assay, (D) flow cytometry and (E) wound healing assay were used to detect cell proliferation, apoptosis, and migration in AGS cells transfected with oe-NC/sh-NC/oe-NSUN2/sh-EPYC (n=3). A-E, two-way ANOVA. All experiments were performed in triplicate.