Additional file 1 of Biofilm communities above and below the cuff of endotracheal tubes are spatially homogenous

Additional file 1. Additional details of methods. Additional Figures: Figure S1 Endotracheal tube (ETT) sectioning guide and assessments. (A) Four cross-sectional sections (1-cm lengths labelled A, B, C and D) from the subglottic region above or the distal region below the cuff margin were excised. Sections were divided longitudinally into two pieces for subsequent analyses (eight segments in total). (B) The number of ETTs included and excluded in the subsequent assessments - scanning electron microscopy (SEM), extended-quantitative culture, and Illumina MiSeq 16S rRNA marker-gene sequencing. *Five participants, whose ETT was assessed, were intubated for