Additional file 1 of Comprehensive assessment of activity, specificity, and safety of hypercompact TnpB systems for gene editing

Additional file 1: Figure S1. gRNA design for all tested target sites. Figure S2. The cleavage mechanism detected by PEM-seq. Figure S3. Typical deletional products of different nucleases. Figure S4. The insertion length distribution and plasmid junction distribution of different nucleases. Figure S5. PEM-seq detected genome-wide editing off-targets of various nucleases. Figure S6. Sequence alignments and reads counts of PEM-seq detected genome-wide editing off-targets of various nucleases at TET3 locus. Figure S7. GUIDE-seq detected genome-wide editing off-targets of different nucleases.