Morphometric Analysis of Adult (P30) Ventricular Cardiomyocytes Following Cardiomyocyte-Specific PKN2 Deletion

This file contains single-cell morphometric measurements of isolated adult ventricular cardiomyocytes at postnatal day 30 (P30). Each row corresponds to one segmented cardiomyocyte and includes sample identifiers (animal and group), image/file provenance, CellProfiler-derived shape features (area, major/minor axis, eccentricity, Feret diameters, radius metrics), and calibrated size readouts in microns (Area_micron, MajorAxis_micron, MinorAxis_micron).Identifiers / indexingUnnamed: 0: CSV export row index (not biological).ImageNumber: CellProfiler image ID used to segment this cell.ObjectNumber: CellProfiler object ID within ImageNumber.QC / classificationclass: CellProfiler Analyst class label (used to keep valid CMs and exclude artifacts/hypercontracted cells).class_number: Numeric encoding of class.Sample & acquisition metadataMetadata_Animal: Animal ID (biological replicate) from image metadata.Animal: Cleaned/standardized animal ID used for analysis/modeling.Group: Experimental group (e.g., Control vs cKO).Metadata_type: Condition/type label embedded in metadata (often overlaps with Group or genotype label).Metadata_Channel: Imaging channel identifier used for segmentation/measurement.Metadata_FileLocation: Source file location string from acquisition/processing.Metadata_Frame: Frame index (if multi-frame/time series).Metadata_Series: Series index (if multi-series acquisition).File provenance (traceability to raw images)FileName_Cells: Image filename used for cell segmentation.FileName_Nucleus: Image filename for nuclear channel (if present/linked).PathName_Cells: Path to the cell image.PathName_Nucleus: Path to the nuclear image.Morphometrics (CellProfiler AreaShape_; typically in pixel units unless otherwise stated)AreaShape_Area: Segmented cell area.AreaShape_BoundingBoxArea: Area of minimal bounding rectangle around the cell.AreaShape_Compactness: Compactness/shape irregularity metric (higher = less compact/more irregular).AreaShape_ConvexArea: Area of the convex hull of the cell.AreaShape_Eccentricity: Ellipse eccentricity (0=circle, 1=highly elongated).AreaShape_MajorAxisLength: Major axis length of best-fit ellipse (cell “length”).AreaShape_MinorAxisLength: Minor axis length of best-fit ellipse (cell “thickness/width”).AreaShape_MaxFeretDiameter: Maximum Feret (caliper) diameter.AreaShape_MinFeretDiameter: Minimum Feret (caliper) diameter.AreaShape_MaximumRadius: Max centroid-to-boundary distance.AreaShape_MeanRadius: Mean centroid-to-boundary distance.AreaShape_MedianRadius: Median centroid-to-boundary distance.Calibrated outputs (micron units)Area_micron: Cell area in µm² (calibrated from AreaShape_Area).MajorAxis_micron: Cell length in µm (calibrated from AreaShape_MajorAxisLength).MinorAxis_micron: Cell thickness/width in µm (calibrated from AreaShape_MinorAxisLength).