Statistical Analysis Script for Embryonic Cardiomyocyte Proliferation (EdU) in PKN2 cKO and Control Hearts

This Quarto (.qmd) script performs the statistical analysis and visualization of embryonic cardiomyocyte proliferation data (EdU incorporation) comparing PKN2 cardiomyocyte-specific knockout (cKO) and control hearts. The workflow imports per-field EdU count data, derives proliferative fractions, and fits generalized linear mixed-effects models (glmmTMB) with animal as a random effect and developmental stage as a fixed factor. The script generates model-based estimates, pairwise comparisons, and publication-ready plots corresponding to the proliferation analyses presented in the manuscript.