Additional file 2 of Pre-therapeutic bone marrow-resident leukemic cells in acute myeloid leukemia exhibit a distinct dysregulated calcium signature and stem-like profile reflecting minimal residual disease precursors

Supplementary Material 2: Figure S1. Identification and phenotypic characterization of rare BMresLC in xenografted mouse BM. (A) Heatmap showing, among total murine BM cells, the frequency of human BMresLC expressing the indicated surface markers. Human B2Mpos cells are shown in pink, B2Mpos/Ki67pos cells in green, and CD33pos myeloid leukemic cells in blue. Samples were annotated by patient identifier (AML#) and by mouse replicate (letter), such that each xenografted mouse derived from the same patient sample is uniquely labeled (B) Representative flow cytometry plots illustrating the gating strategy for BMresLC (Red A780-60neg viable cells) and confirming human myeloid identity through hCD33 expression, compared with diagnostic AML blasts. (C) Representative flow cytometry plots showing Ki67 expression within BMresLC gated on hCD33pos cells. (D) Quantification of Ki67low slow-cycling cells in diagnostic AML samples compared with BMresLC, highlighting the slow-cycling phenotype of residual BM populations. Statistical significance was performed using Student’s t test (** p < 0.01). (E) Percentage of viable cells (Red A780-60neg) in diagnostic AML samples versus BMresLC, demonstrating the enhanced survival capacity of BM-resident leukemic cells under identical ex vivo conditions. Statistical significance was performed using Student’s t test (** p < 0.01).