Additional file 4 of Pre-therapeutic bone marrow-resident leukemic cells in acute myeloid leukemia exhibit a distinct dysregulated calcium signature and stem-like profile reflecting minimal residual disease precursors

Supplementary Material 4: Figure S3. Expression of SOCE pathway actors shows isoform-dependent variation according to disease stage. (A) Relative ORAI1, ORAI2, and ORAI3 gene expression in BMresLC and AML cells at the diagnosis stage was determined by RT-qPCR. ORAI1, ORAI2, and ORAI3 relative gene expression was calculated by the 2-ΔΔCt method and normalized with the isoform ORAI1 value (diagnosis). Statistical significance was performed using Student’s t test (* p < 0.05). (B) Relative STIM1, STIM2 gene expression in BMresLC and AML cells at the diagnosis stage was determined by RT-qPCR. STIM1, STIM2 relative gene expression were calculated by the 2-ΔΔCt method, normalized with the isoform STIM1 value (diagnosis). Statistical significance was performed using Student’s t test (* p < 0.05). (C) Relative NFATc1, NFATc2, NFATc3, and NFATc4 expression in BMresLC and AML cells at diagnosis determined by RT-qPCR. NFATc1, NFATc2, NFATc3, and NFATc4 relative expression was calculated by the 2-ΔΔCt method and normalized with the isoform NFATc1 value (diagnosis). Statistical significance was performed using Student’s t test (* p < 0.05, *** p < 0.001).