Additional file 1 of Towards the development of a CRISPR-Cas9 based kill switch for Saccharomyces cerevisiae

Supplementary material 1: Figure S1. Fluorescence microscopy images of selected strains. Figure S2. Growth profiles and the generation time at the maximum specific growth rate plotted against the lag time of KiSS strains with single gRNA targets at different ATc concentrations. Figure S3. Growth profiles of KiSS strains with single gRNA targets without induction. Figure S4. Growth profiles of experimental replicates of KiSS strains with single gRNA targets with and without induction. Figure S5. Growth profiles of experimental replicates of KiSS strains with single or dual gRNA targets without ATc. Figure S6. Escape frequency of cultures without induction. Table S1. List of oligos used. Table S2. Plasmids used and developed. Table S3. Sequencing results of selected KiSS strains.