Original data of the paper

Background: Mitochondrial permeability transition-driven necrosis (MPTDN) hasbeen implicated in a variety of diseases, but its relationship with colorectal cancer(CRC) prognosis is unclear.Methods: In this study, TCGA-COAD and TCGA-READ datasets were analyzed toidentify differentially expressed genes (DEGs) in CRCs. Differentially expressedMPTDNGRs (DE-MPTDNRGs) were identified by comparing DEGs to MPTDNrelatedgenes (MPTDNRGs). Univariate Cox, Minimum Absolute Contraction andSelection Operator (LASSO) regression and risk scoring analysis divided TCGACRCpatients into high- and low-risk cohorts.Results: The prognostic genes LMNB2, CASP7, PRKCB, GZMB and ENDOG wereidentified, and the survival rate of high-risk patients was poor. Independentprognostic factors, including risk score, age, and N stage, are effectivepredictors of survival. Immunoassays revealed that high-risk patients had 9elevated immune checkpoints, while low-risk patients were more susceptible topazopanib and temsirolimus. In addition, single-cell analysis showed that PRKCBand GZMB were highly expressed in stem cells, while LMNB2 was moreabundantly expressed in mast cells. Real-time PCR (RT-qPCR) confirmed lowlevels of CASP7, PRKCB, and ENDOG mRNA in CRC tissues, with no significantdifference between LMNB2 and GZMB.Conclusion: These findings highlight 5 MPTDN-associated prognostic genes inCRC, providing insights for individualized treatment and prognosis.