Additional file 1 of Simplified flow cytometric quantification of human neutrophil extracellular traps (NETs)

Supplementary Fig. 1 Flow cytometric detection and quantification of uplifted NETs post monolayer culture in unfixed cells. (A) Representative flow cytometry plots and images of NETotic cells post-monolayer culture after 3 h of incubation. Scale = 10 μm. (B) Quantification of mean percentages of double positive H1-DNA/H2A cells. (C) Representative flow cytometry plots of unfixed NETotic cells after 3 h of incubation. (D) Quantification of mean percentages of double positive Sytox green/H2A cells. Data are expressed as means +/- SEM, n = 3 distinct cell donors. **P < 0.01 vs. control, ##P < 0.01 vs. PMA