Additional file 2 of Simplified flow cytometric quantification of human neutrophil extracellular traps (NETs)

Supplementary Fig. 2 Fixed 3-colour flow cytometric detection and quantification of established PMA-induced NETosis. (A) Representative flow cytometry plots of NETotic cells identified using fluorescent markers for H1-DNA, H2A and MPO. (B) Quantification of mean percentages of double positive H1-DNA/H2A cells. (C) Quantification of mean percentages of double positive H1-DNA/MPO cells. Data are expressed as means +/- SEM, n = 3 distinct cell donors. **P < 0.01 vs. control, ##P < 0.01 vs. PMA