Determination of anti-HIV antibodies by Determine HIV-1/2 (Abbott).

Determine (TM) HIV-1/2 is an immunochromatographic test for the qualitative detection of antibodies to HIV-1 and HIV-2. Sample (serum, plasma, or whole blood by venipuncture) is added to the sample pad. As the sample migrates through the conjugate pad, it reconstitutes and mixes with the selenium colloid-antigen conjugate. This mixture continues to migrate through the solid phase to the immobilised recombinant antigens and synthetic peptides at the patient window site. If antibodies to HIV-1 and /or HIV-2 are present in the sample, the antibodies bind to the antigen-selenium colloid and to the antigen at the patient window, forming a red line at the patient window site. If antibodies to HIV-1 and/or HIV-2 are absent, the antigen-selenium colloid flows past the patient window, and no red line is formed at the patient window site. To insure assay validity, a procedural control bar is incorporated in the assay device.