Additional file 2 of Effects of the type V secretion system eYadA on the biological properties and pathogenicity of avian pathogenic Escherichia coli

Additional file 2. Analysis of the sequence and Phylogenetic tree for YadA. A. Sequence analysis of YadA. The YadA sequences from Yersinia enterocolitica, Yersinia pseudotuberculosis, and Escherichia coli were retrieved from the National Center for Biotechnology Information (NCBI) and used for sequence analysis. A substantial number of consecutive identical YadA sequences are observed across various strains (such as blue, cyan, and other regions characterized by uniform color blocks), indicating that these areas have remained highly conserved throughout evolutionary history. B. Phylogenetic tree analysis of YadA. A phylogenetic analysis of the YadA protein sequences was conducted using MEGA 7.0 [38]. Initially, the ClustalW algorithm was employed for multiple sequence alignment, followed by manual refinement of conserved loi. The phylogenetic tree was constructed utilizing the Neighbor-Joining (NJ) method. The reliability of the branches was assessed through bootstrap analysis with 1000 repeated samplings. The target sequence (eYadA) demonstrates the highest homology with Escherichia coli YadA.