Additional file 3 of Effects of the type V secretion system eYadA on the biological properties and pathogenicity of avian pathogenic Escherichia coli

Additional file 3. H&E staining of pathological tissue sections from infected mice. Following intraperitoneal infection with a concentration of 1 × 108 CFUs (100 μL) of APEC TW-XM or APEC TW-XMΔeyadA, mice were observed for a duration of 12 h before the preparation of tissue samples for paraffin-embedding and subsequent hematoxylin and eosin (H&E) staining. Histopathological changes were examined using microscopy (BX53, Olympus Corporation, Japan).