Additional file 1 of Foot-and-mouth disease virus VP4 interferes with host interferon response by targeting the nuclear translocation of interferon regulatory factor 3 (IRF3)

Additional file 1. VP4 protein expression on cell lines. Expression of the Flag-VP4 stably expressed in the A 3D4/21 cells, transiently expressed Flag-VP4 in B PK15 cells, C LFBK cells, and D HEK293T cells was evaluated by immunoblotting. Cells underwent transfection with either Flag-VP4 or a control vector. Subsequently, cellular RNA was isolated. Transcription of Flag-VP4 was subsequently analyzed in E 3D4/21 cells, F PK15 cells, G LFBK cells, and H HEK293T cells by qRT-PCR. All qRT-PCR data represent at least two independent experiments, each with similar results, and the values are expressed as the mean ± SD of three biological replicates. All the immunoblot data represent at least two independent experiments, each with similar results. Student’s t-test; *p < 0.05; **p < 0.01; ***p < 0.001.