Additional file 2 of Foot-and-mouth disease virus VP4 interferes with host interferon response by targeting the nuclear translocation of interferon regulatory factor 3 (IRF3)

Additional file 2. Screening result of FMDV proteins and interaction of VP4 with KPNA2 and KPNA4. A HEK293T cells underwent co-transfection with RIG-I, TK Renilla, and IFN-β promoter luciferase reporter plasmids and the plasmids containing structural proteins of the FMDV genome. Subsequently, RLR-mediated IFN-β promoter activity was evaluated. HEK293T cells underwent co-transfection with Strep-VP4 and B Flag-KPNA2 or C Flag-KPNA4 plasmids, and lysates underwent an immunoprecipitation assay and immunoblotting. Data represent a minimum of two independent experiments, each presenting similar results, and the values are expressed as mean ± SD of three biological replicates. All the immunoblot data are representative of at least two independent experiments, each with similar results. Student’s t-test: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.