Ratiometric imaging of cells stained with the solvatochromic probe JONR

This dataset presents representative ratiometric fluorescence imaging results obtained from cells stained with the solvatochromic probe JONR. The ratiometric signal reflects variations in lipid membrane polarity and enables visualization of membrane heterogeneity within living cells. Differences in emission ratios correspond to changes in the local membrane microenvironment, allowing the identification of distinct subcellular membrane structures.