Additional file 5 of Consequences of irradiation on blood-brain tumor barrier model of Diffuse Midline Glioma: characterization of physical and metabolic properties

Supplementary Material 5: Additional file 4. Investigation of the effects of irradiation on physical properties of the BBTB-013. (A) BBTB-013 ECs permeability to Lucifer Yellow 24 h and 7 days after irradiation (2, 4 and 6 Gγ). (Pe = endothelial permeability coefficient). One-way ANOVA followed by Dunnett’s post-hoc test, ns = non-significant, compared to the non-irradiated control (N = 3; n = 9). (B) Representative images of adherent junctions VE-cadherin and tight-junction-associated proteins zonula occludens-1 (ZO-1), 7 days after 6 Gγ of irradiation. Scale bar = 25 µm. (C) & (D). Protein levels of Claudin-5 and ZO-1 in BBTB-013 ECs, 7 days after irradiation at a dose of 6 Gy, quantified by Western blot and normalized to GAPDH protein level. The line represents the non-irradiated control. Unpaired t-test was used for statistical study, (N = 3)