Additional file 4 of Systematic perturbation of cultures of K. phaffii by carbon co-feeding show gene signatures associated with production of recombinant proteins

Supplementary Material 4. Figure S1: SDS-page gel of 3 mL GFP cultivation in 20 different co-feeding environments. Figure S2: Correlation of gene expression between AOX1 and DAS2 promoter systems. Figure S3: Effect of Systematic Perturbation on Final Culture Density and Recombinant Protein Titer of VHH Using Carbon Co-feeding. Figure S4: Effect of Systematic Perturbation on Final Culture Density and Recombinant Protein Titer of IgG1 Using Carbon Co-feeding. Figure S5: Statistical Significances of Co-feed Experiments on Titers. Figure S6: Statistical Significances of Co-feed Experiments on Biomass (OD600). Figure S7: Statistical Significances of Co-feed Experiments on Ratio of Protein to Biomass. Figure S8: Elbow Plots to determine required sparsity and number of components. Figure S9: Correlation of all metagenes with specific productivity. Figure S10: Relationship of Metagene 3 with Transgene Expression. Figure S11: Operational Parameters and Cellular Phenotypes of 1-L Perfusion Reactor Cultivation.