Additional file 1 of Ultrasonic repression of TRPA1-dependent astrocyte reactivity confers neuroprotection in models of Lewy body dementia

Additional file 1. Fig. S1. Information on Ultrasonocoverslip. Fig. S2. Ultrasound and TRPA1 inhibition suppress astrocytic inflammatory responses induced by conditioned media from α-synuclein PFF–exposed BV2 cells and by direct PFF treatment, as measured by RT-qPCR. Fig. S3. Conditional α-synuclein (A53T) Tg transgenic mice have no neuropathologies in brains with normal behavior. Fig. S4. Ultrasound intensity-dependent brain pathologies in wild type mice. Fig. S5. Hippocampal PFF injection in α-synuclein transgenic mice induces Lewy pathology spread to the cortex, but not to the ventral midbrain, within 1 month. Fig. S6. Transcranial ultrasound application represses hippocampal TLR2 upregulation, microgliosis, and neurodegeneration in α-synuclein transgenic mice with hippocampal α-syn preformed fibril (PFF) injection. Fig. S7. Schematic illustration of ultrasound-mediated astrocyte repression and neuroprotection in LBD brains. Fig. S8-1. Full, uncropped original brain section images corresponding to main and supplementary figure panels. Fig. S8-2. Full, uncropped original brain section images corresponding to main and supplementary figure panels. Table S1. Sequences of primers used for RT-qPCR.