Additional file 1 of Gut-initiated alpha synuclein fibrils drive parkinsonism phenotypes: temporal mapping of REM sleep behavior disorder-like and other non-motor symptoms

Additional file 1. Fig. S1 Confocal images of transverse sections of the stomach 1 month following injections at 2 different levels (α and β) showing staining of pS129-αSyn, ChAT, TH, and DAPI. Fig. S2 Semi-automated whole brain mapping of pSer-129-αSyn staining. Fig. S3 Behavioral results of control groups. Fig. S4 Detailed effects of αSyn-PFF injections on sleep parameters. Fig. S5 Detailed effects of αSyn-PFF injections on non-motor behavioral tests. Fig. S6 Detailed effects of αSyn-PFF injections on motor behavioral tests. Fig. S7 Detailed effects of αSyn-PFF injections on cognitive behavioral tests. Fig. S8 Detailed sex effects of αSyn-PFF injections in multiple brain regions and in behavioral tests. Fig. S9 Detailed behavioral effects of αSyn-PFF injections in striatum or SNc. Fig. S10 Detailed effects of αSyn-PFF injections in striatum or SNc on sleep parameters.