Additional file 3 of Cell-intrinsic vulnerability and immune activation cooperate to drive degeneration in a mitochondrial complex I deficiency model of optic neuropathy

Supplementary Material 3. Supplementary Fig S3. Activated myeloid cells are present in the center and periphery of α-Cre; Ndufs4fl/fl retinas. Retinal flat-mounts from P45 α-Cre; Ndufs4fl/fl mice immunostained for Iba1 (magenta) and Cd68 (teal) reveal a high density of activated myeloid cells across both central and peripheral regions of the GCL. Iba1⁺ cells co-express Cd68, a marker of activation and phagocytic activity, indicating widespread microglial activation in the degenerating retina.