Additional file 3 of A recombinant adenoviral vector vaccine expressing the ORF2 capsid protein confers robust protection against chicken astrovirus infection

Additional file 3: The original images of the gel electrophoresis and western blot for Figure 2. Figure 2B Western blot analysis of rAd5-CAstV-ORF2 expression; Figure 2C Genetic stability of rAd5-CAstV-ORF2: Upper panel: 293 T cells infected with rAd5-CAstV-ORF2 were passaged for 10 generations. Genomic DNA from generations 2, 4, 6, 8, and 10 was extracted to assess ORF2 gene stability. Lower panel: Cells from each generation were lysed, proteins extracted, and western blot analysis confirmed ORF2 protein stability.