Slow Intrinsic Oscillations in the Ventrolateral Preoptic Nucleus

The following data set concerns the article:“Slow Intrinsic Oscillations in the Ventrolateral Preoptic Nucleus”Authored by: Perrenoud Q., Ribot J., Geoffroy H., Gallopin T., Rouach N. and Rancillac A. Question and request should be addressed to Armelle Rancillac: armelle.rancillac@college-de-france.fr The ventrolateral preoptic nucleus (VLPO) of the hypothalamus plays a major role in the induction and consolidation of non-rapid eye movement (NREM) sleep. While VLPO neurons are heterogeneous, they often display low-threshold spikes (LTS), a feature that supports rhythmic activity. In the connected article, we report that some VLPO neurons display spontaneous rhythmic bursting of action potentials. This activity occurred in putative sleep-promoting neurons, identified by inhibitory responses to noradrenaline (NA), as well as wake-active neurons that were activated by NA. Unsupervised clustering of 24 bursting neurons based on burst properties, electrophysiological, and morphological features revealed three distinct groups: one corresponding to putative sleep-promoting neurons and two wake-active neurons with fast and slow bursting dynamics. The present data set contains a data table (Input_Matrix.xlsx) and matlab scripts operating on this data table, to generate the figure panels related to the clustering of bursting VLP neurons in the connected article.