Peptidomelanin release assays - underlying data

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Authors: Deepesh Nagarajan, Pema Lhamu, Spoorthi R. Kembhavi
Cited by: 1

Abstract

################################################################################ Peptidomelanin Release Analysis and Figure Generation: This repository contains scripts used to analyze melanin release experiments and generate publication-quality figures. The workflow processes experimental absorbance measurements, performs statistical analysis, and produces figure panels suitable for inclusion in manuscripts.This underlying data contains the following files (besides README):-Melanin_release.csv: file containing all the raw data for melanin release assays.-Panel_A.R: R scripts for image generation and statistical analysis of Figure 1A in the main article. Generates the time-course plot of melanin release comparing two fungal strains.. This R script draws input from Melanin_release.csv.-Panel_B.R: R scripts for image generation and statistical analysys for Figure 1B in the main article. Generates a comparison of melanin release across different nutrient conditions. This R script draws input from Melanin_release.csv.-commands.sh: A bash/shell wrapper script that runs Panel_A.R and Panel_B.R. It will concatenate the image outputs and generate a combined figure (panel_AB.png).-release.png: Same as panel_AB.png but I manually added annotations (panel labels: A and B). This is the final image that is included in the article.-panel_A.pdf, panel_B.pdf, panel_A.png, panel_B.png, panel_AB.png are intemediate outputs.################################################################################ DEPENDENCIES: These scripts were run on Ubuntu 22.04.5 LTS (jammy)bash scripting: Required for installing R, needed for stat. anal. and img. gen.: $ sudo apt install r-base-core bash scripting: Required for installing pdf2ppm, for converting pdfs to pngs: $ sudo apt install poppler-utils bash scripting: Required for combining images: $ sudo apt install imagemagick R scripting: install beeswarm package, needed for jittering datapoints: >install.packages("beeswarm")

Publication details

Journal / source
Figshare
Publication date
13 March 2026
Document type
Dataset