Ambystoma barbouri larval transcriptome annotation

We assembled and annotated the first de novo transcriptome of Ambystoma barbouri from larval individuals exposed to two temperature treatments as well as adult heart, liver, spleen, and tail tissue samples. RNA reads were assembled with Trinity and rnaSPAdes, and merged using EvidentialGene followed by CD-HIT-est. This produced a transcriptome with 850,204 unigenes. The assembly possessed 98.1% of complete vertebrate BUSCO markers, an N50 of 1,628 bp, and an L50 of 130,416. TransDecoder identified 534,476 protein coding sequences, of which 276,770 possessed annotation information within one or more of the applied databases (EggNM, GO, Infernal, KEGG, Ko Fam, Pfam, SignalP, SwissProt). This assembly provides a reference for gene expression and population-level studies as well as conservation genetics within Ambystoma and in amphibians.