Pathological phospho-tau tangle and amyloid-beta deposit formation in CSE-deficient mouse brain.

Figure 1. Pathological phospho-tau tangle formation in CSE-deficient mouse brain. Representative confocal images of sagittal cortical brain sections from 18-month-old wild-type (WT) and CSE-/- mice immunostained with the phospho-tau antibody PHF13 (pSer396, Cell Signalling, Cat. No. 9632S, 1:50), followed by goat anti-mouse IgG-CFL488 secondary antibody (Santa Cruz Biotechnology, Cat. No. sc-362257, 1:200). Representative images from n = 3 animals per genotype are shown. Scale bar, 30 μm.Figure 2. Increased pathological phospho-tau accumulation in CSE-deficient mouse brain. a, Representative confocal images of sagittal cortical brain sections from 18-month-old wild-type (WT) and CSE-/- mice immunostained with the phospho-tau antibody AT8 (pSer202/pThr205; Thermo Fisher Scientific, Cat. No. MN1020, 1:50), followed by goat anti-mouse IgG-CFL488 secondary antibody (Santa Cruz Biotechnology, Cat. No. sc-362257, 1:200). Nuclei were counterstained with DAPI. Representative images from n = 3 animals per genotype are shown. Scale bar, 20 μm. b, Immunoblot analysis of phospho-tau (AT8, pSer202/pThr205) in whole-brain lysates from 10-month-old WT and CSE-/- mice. Quantification of phospho-tau levels is shown on the right. Data are presented as mean ± s.d. (n = 4 animals per group). Statistical significance was determined using a two-tailed unpaired Student's t-test.Figure 3. Detection of amyloid-beta deposits in CSE-deficient mouse brain. Representative confocal images of sagittal cortical brain sections from 18-month-old wild-type (WT) and CSE-/- mice immunostained with the amyloid-beta antibody (Santa Cruz Biotechnology, Cat. No. sc-28365, 1:50), followed by goat anti-mouse IgG-CFL488 secondary antibody (Santa Cruz Biotechnology, Cat. No. sc-362257, 1:200). Representative images from n = 3 animals per group are shown. Scale bar, 30 μm.