3D microscope image acquisition method based on liquid lens with translational field of view

Three-dimensional (3D) microscopic imaging techniques are facing challenges to achieve greater imaging depth and enhanced visualization of results. In this paper, we propose a 3D microscope acquisition method based on a liquid lens with the translational field of view (LLTFOV). The method utilises the fast zoom function of the LLTFOV to achieve rapid depth focusing of the microscope, obtain parallax images of the specimen with complete depth information, and improve the accuracy and reliability of 3D reconstruction. By utilizing the LLTFOV's precise field of view (FOV) adjustment function, the parallax image of the specimen with multi-angle, uniform, and no vertical parallax is obtained, and the visualisation effect of the 3D image of the specimen is improved. The 3D image of the specimen is obtained by displaying the acquired parallax synthesis image using a 3D display screen. The experimental results demonstrate that the method can efficiently reproduce the 3D images of the specimens, improving the image quality and visual experience, making it easier to obtain high-quality 3D images in scientific research and applications.