Highly fluorescent method for ultra-sensitive estimation of delafloxacin in human plasma: greenness assessment

Abstract A simple, rapid, sensitive, and reliable spectrofluorimetric approach has been developed for the analysis of delafloxacin (DEL) in human plasma. The proposed method is based on the formation of a micellar fluorescent complex through the interaction of DEL with 2% sodium dodecyl sulfate (SDS) using a phosphate buffer (pH 4.2), producing a pronounced emission at 470 nm upon excitation at 380 nm. The experimental conditions that affected the one-pot synthesis were investigated and optimized. The calibration curve exhibited a linear relationship over a concentration range of 2.0–120.0 ng mL–1, with detection (LOD) and quantitation limits (LOQ) 0.6 and 1.9 ng mL–1, respectively. The designed approach was validated following the ICH and FDA guidelines, producing a precise analysis of DEL in its tablets and plasma samples with high recovery percentage. The results provide that the method possesses excellent eco-friendly properties, highlighting its strong compatibility with the principles of green chemistry.