Prevalence of Gram-negative Bacterial Pathogens in Populations Suffering from Urinary Tract Infections (UTI) in the City of Sirte.

Background: Urinary tract infections (UTIs) are a prevalent ailment on a global scale. The occurrence of urinary tract infections (UTIs) in both men and women has been associated with unusual bacterial growth. The aim of the present study is to identify and characterise gram-negative bacteria that are capable of causing urinary tract infections. Methodology: Midstream urine samples were collected from each patient into sterile, screw-capped universal container and inoculated into CLED agar plates subsequently placed in an incubator at 37°C for a period of 24 hours. A series of biochemical tests were conducted using Analytical Profile Index (API. ( Results: a total of 150 samples were isolated and subjected to rigorous investigation. A total Out of 96 samples were isolated and identified as gram-negative bacteria, and the most prevalent isolated bacteria were Escherichia coli, with 51 cases (53.1%), followed by Klebsiella spp. in 17 cases (17.7%), Pseudomonas in 12 cases (12.5%), Acinetobacter in 9 cases (9.4%), and Enterobacter in 7 cases (7.3% ( Conclusions: The present study revealed a widespread prevalence of urinary tract infections caused by Gram-negative bacterial species, with E. coli being the most virulent, particularly in women and children. Keywords: Urinary tract infections, Gram negative bacteria, CLED agar, MacConkey agar.