Genome-wide investigation of WRKY gene family in Lagenaria siceraria

Abstract Background A significant family of transcription factors known as WRKY genes include many physiological functions and environmental adaptations. However, insufficient information was previously available about the WRKY genes in Lagenaria siceraria , a crucial crop with substantial economic significance. The recent publication of the whole-genome sequence of L. siceraria has allowed us to perform a genome-wide investigation of the organization of the WRKY genes in L. siceraria . Results In the present study, 57 L. siceraria WRKY ( LsiWRKY ) genes were identified and given new names based on their relative chromosomal distribution. The 57 LsiWRKYs were further divided into three major groups and several subgroups based on their structural and phylogenetic properties. Segmentation duplication events have played a major role in the expansion of the WRKY gene family in L. siceraria . Phylogenetic comparisons of the Group III WRKY genes provide valuable insights into the evolutionary characteristics of WRKY genes in L. siceraria . Additionally, RNA-seq analysis revealed distinct expression pattern of WRKY genes across different tissues. Conclusions This study presents a preliminary analysis of the WRKY gene family in L. siceraria , including their structural characteristics, evolutionary traits, and tissue-specific expression patterns. The systematic insights provided here serve as a foundation for further functional studies aimed at enhancing L. siceraria crops. This knowledge holds promise for improving the cultivation and yield of L. siceraria , thereby contributing to agricultural advancements.