Unraveling Subcellular Ultrastructure with Cyclically Multiplexed Expansion Microscopy

bioRxiv (Cold Spring Harbor Laboratory) 2026 Preprint Open access

Read full text
Authors: Seweryn Gałecki, Bo-Jui Chang, Felix Yuran Zhou, Qionghua Shen, Daniel Stoddard, Bingying Chen, Daniela Nicastro, Reto Paul Fiolka, Kevin M. Dean
DOI: 10.64898/2026.01.01.697161
Cited by: 0
References: 27

Abstract

Despite advances in fluorescence microscopy, spectral overlap and limited resolution hinder the dense mapping of the cellular ultrastructure. To overcome these challenges, we developed Cy-ExM, a high-plex imaging strategy that integrates optimized cryo-fixation for antigen preservation, expansion microscopy, and iterative immunofluorescence labeling. Using oblique plane microscopy, we perform three-dimensional super-resolution imaging of 20 biological targets encompassing the full cellular volume of individual mammalian cells.

Fields of Study & Subjects

Advanced Fluorescence Microscopy Techniques Digital Holography and Microscopy Advanced Electron Microscopy Techniques and Applications

Publication details

Journal / source
bioRxiv (Cold Spring Harbor Laboratory)
Publication date
2 January 2026
Language
EN
Document type
Preprint