FUSION: A fast and uniform processing framework for whole-brain optical scattering tomography images

Abstract Coherent scattering imaging has seen substantial development in clinical ophthalmology and endoscopic microscopy owing to its high throughput, large dynamic range, and capability for label-free imaging. However, limited optical penetration has constrained its application to whole-organ imaging studies. Here, we propose a high-throughput coherent scattering imaging system assisted by tissue clearing, termed fu CAST, which enables whole-brain white-matter imaging. In parallel, we develop a high-throughput image reconstruction and uniform stitching platform, FUSION, specifically tailored for fu CAST datasets.