Analytical Sensitivity of the BOSCH Vivalytic Molecular Assay for Detection of Monkeypox Virus Clade Ib

medRxiv 2026 Preprint Open access

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Authors: Camille Escadafal, Kenneth Adea, Placide Mbala, Prof. Dr. MD Isabella Eckerle
DOI: 10.64898/2026.01.07.25342807
Cited by: 0
References: 5

Abstract

Abstract We evaluated the BOSCH Vivalytic MPXV assay using serial dilutions of monkeypox virus clade Ib, a new offshoot of clade I identified in 2023. The assay detected viral DNA down to ∼100 copies/mL demonstrating comparable analytical sensitivity to our in-house reference PCR and to other commercial platform-based mpox molecular assays.

Fields of Study & Subjects

Poxvirus research and outbreaks Biosensors and Analytical Detection Virology and Viral Diseases

Publication details

Journal / source
medRxiv
Publication date
11 January 2026
Document type
Preprint