Mosquitoes of Macha – new mosquito species records from Macha and surrounding locations in southern Zambia with photographs

malaria vectors has been understudied in many parts of Africa. Here, we provide a new species record of mosquitoes from Macha and its surrounding locations in southern Zambia, as well as an updated list of species from the region. With the addition of 19 new species records in this region, the total list of mosquito species reported in Macha is now 46. We present high-resolution focus-stacked photographs of some of these species, many of which were only previously available as text or drawing descriptions in mosquito identification books and articles. We also include 34 COI sequences from Zambian mosquito species, five of which had no prior genetic information on Genbank. This visual guide as well as additional COI sequences provide essential tools for accurate mosquito surveillance in southern Zambia and establishes a baseline data for future invasive species monitoring.