Fusion gene discovery in single cells from high throughput long read single cell transcriptomes
Abstract
High throughput long-read single cell RNA sequencing allows for fusion gene discovery in thousands of cells in parallel, which is however constrained by the lack of robust computational tools. Here, we develop LongFUSE that employs the XOR logic operation and stringent filtering criteria for the accurate detection of cell-specific fusion genes and their splicing isoforms. LongFUSE outperforms existing tools in both simulation and real data, demonstrating high accuracy and robustness.
Fields of Study & Subjects
Single-cell and spatial transcriptomics
RNA Research and Splicing
Cell Image Analysis Techniques
Publication details
- Journal / source
- bioRxiv (Cold Spring Harbor Laboratory)
- Publisher
- openRxiv
- Publication date
- 14 January 2026
- Language
- EN
- Document type
- Preprint