Ultra-fast sample-to-sequencing workflow for clinical diagnostics using micropillars

Abstract We present a streamlined, solid-phase workflow for Oxford Nanopore sequencing that integrates DNA extraction, purification, and library preparation within a single microfluidic cartridge. By eliminating tube transfers and performing all enzymatic steps directly on captured DNA, the method minimizes sample loss, reduces hands-on time, and simplifies library generation for long-read sequencing. Starting from volumes as small as a single drop of blood, this integrated approach produces high-quality sequencing libraries from cell lines, whole blood, and tissue. The workflow achieves robust recovery of high-molecular-weight DNA and high pore occupancy, enabling rapid, low-complexity sample preparation suitable for clinical, field, and decentralized sequencing applications.