Continuous mass photometry by single molecule trapping

bioRxiv (Cold Spring Harbor Laboratory) 2026 Preprint Open access

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Authors: Dan Loewenthal, Roi Asor, Stephen Austen Thorpe, Jack S. Peters, Konstantin Zouboulis, Merve Kaplan, Jan Christoph Thiele, Jack Bardzil, Seham Helmi, Sean Anthony Burnap, Tiong Kit Tan, Weston B. Struwe
+2 more Justin LP Benesch, Philipp Kukura
DOI: 10.64898/2026.02.02.703204
Cited by: 1
References: 22

Abstract

Abstract The dynamic choreography of biomolecular interactions underpins the processes of life, but its direct observation remains challenging. Here, we introduce confined diffusion mass photometry, enabling hour-long, mass-resolved observation of individual biomolecules, their complexes and interactions with up to sub-kDa mass precision and ms temporal resolution. Our approach represents a quantitative time-resolved single-molecule measurement modality for studying complex biomolecular mechanisms in action.

Fields of Study & Subjects

Advanced Fluorescence Microscopy Techniques Microtubule and mitosis dynamics Click Chemistry and Applications

Publication details

Journal / source
bioRxiv (Cold Spring Harbor Laboratory)
Publisher
openRxiv
Publication date
3 February 2026
Language
EN
Document type
Preprint