In vivo experimental model of β-glucan-induced innate immune memory

SUMMARY Innate immune memory is the ability of innate immune cells to develop a recallable, epigenetically imprinted response after an initial stimulus, enabling them to mount differential responses upon restimulation. Here, we present a detailed protocol of β-glucan preparation, and its in vivo application to modulate innate immune memory in mice through repeated intraperitoneal (IP) injections. This approach results in enhanced hematopoiesis and bone marrow specific myeloid bias, key hallmarks of in vivo innate immune memory responses. For complete details on the use and execution of this protocol, please refer to Hassan et al. Graphical abstract Highlights Preparation of β-glucan for in vivo experiments. In vivo modulation of innate immune memory through repeated intraperitoneal β-glucan injections in mice. Modulation of innate immune memory is assessed by enhanced bone marrow hematopoiesis and GMP production.